Expression profile and bioinformatics analysis of circular RNAs in tongue squamous cell carcinoma. (19th June 2021)

• Record Type: Journal Article
• Authors: Chen, Zexi; Jie, Yinghui; Qiu, Zailing; Zhang, Mi; Shi, Bin; Zhu, Xiaofeng; Chen, Jiang
• Is Part Of: Oral diseases. Volume 29:Number 2(2023)
• Page Start: 458
• Page End: 468
• Publication Date: 2021-06-19
• Subjects: biomarkers -- circular RNA -- high‐throughput sequencing -- network -- tongue squamous cell carcinoma; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.13933
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract: Objective: This study was designed to identify the role of circRNAs in tongue squamous cell carcinoma (TSCC) and discover novel circRNAs as potential diagnostic or therapeutic targets for TSCC. Results: The circRNA expression profiles in TSCC were evaluated by high‐throughput sequencing in 6 TSCC patients. Our data showed that 69 circRNAs were downregulated and 208 were upregulated significantly (fold change ≥2.0; p < 0.05). GO and KEGG analysis demonstrated that the parental genes of differentially expressed circRNAs were potentially implicated in TSCC pathogenesis. After bioinformatics analysis, 4 circRNAs (hsa_circ_0005035, hsa_circ_0002360, hsa_circ_0066251, and hsa_circ_0003161) were selected and successfully validated by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) in 40 patients and 3 TSCC cell lines. The establishment and analysis of the circRNA‐miRNA‐mRNA network revealed the potential function and mechanism of these candidate circRNAs. Conclusion: Our study provided a comprehensive circRNAs expression profile of TSCC by RNA‐seq and discovered 4 novel circRNAs with potential great diagnostic and therapeutic value. These findings provide new insights into the development of potential biomarkers and targets for TSCC treatment.