Efficient Enrichment of Global Phosphopeptides Using Magnetic Tannic Acid – Titanium(IV)/Zirconium(IV) Functionalized Spheres as a Novel Sorbent for Immobilized Metal Ion Affinity Chromatography (IMAC). (13th April 2023)

• Record Type: Journal Article
• Authors: Ouyang, Menglin; Wu, Jiani; Yan, Yinghua; Ding, Chuan-Fan
• Is Part Of: Analytical letters. Volume 56:Number 6(2023)
• Page Start: 1016
• Page End: 1030
• Publication Date: 2023-04-13
• Subjects: phosphopeptides -- immobilized metal ion affinity chromatography (IMAC) -- tannic acid -- solid-phase extraction (SPE) nanospheres; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2022.2116644
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Inspired by the enrichment preferences of metal ions for phosphopeptides, a layered assembly method was adopted to introduce hydrophilic polyhydroxy ligands tannic acid (TA) to prepare magnetic bimetal ion-functionalized nanomaterials denoted by Fe3 O4 @TA@Ti 4+ /Zr 4+ to provide the comprehensive enrichment of phosphopeptides. Benefiting from the excellent ion immobilization ability and unbiasedness for phosphopeptide enrichment, Fe3 O4 @TA@Ti 4+ /Zr 4+ shows excellent properties for the enrichment and identification of global phosphopeptides. The results show that the material possesses high efficiency, outstanding selectivity (the maximum molar ratio β-casein to bovine serum albumin is 1:1000), a low detection limit of 0.4 fmol/μL, and the ability to be recycled at least 10 times. In addition, excellent performance was also demonstrated in the analysis of skimmed milk and human saliva: 13 and 31 phosphopeptides were successfully captured. The results demonstrate that the material has high potential for practical application in phosphoproteomics.