An LRR domain-containing membrane protein gene in rotifer Brachionus plicatilis: Sequence feature, expression pattern, and ligands binding activity. (April 2023)

• Record Type: Journal Article
• Authors: Wang, Yuxi; Guo, Ying; Hu, Jingjie; Bao, Zhenmin; Zhou, Bin; Wang, Mengqiang
• Is Part Of: Developmental and comparative immunology. Volume 141(2023)
• Publication Date: 2023-04
• Subjects: Rotifer -- Brachionus plicatilis -- Leucine-rich repeat -- Innate immunity; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2023.104634
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Leucine-rich repeat (LRR) domains mediate multiple innate immune responses via protein-ligand and protein-protein interactions, but their exact roles in invertebrates are poorly understood. Herein, an LRR domain-containing transmembrane protein ( Bp LRRm) was identified in the rotifer Brachionus plicatilis . The 1069 bp Bp LRRm nucleotide sequence contains a 942 bp open reading frame (ORF) encoding a 313 amino acid polypeptide with four LRR motifs harbouring the LXXLXXLXLXXNXLXXL motif, and a transmembrane domain. Treatment with 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47) decreased Bp LRRm mRNA levels at 3 h, but they increased thereafter and peaked at 12 h. Lipopolysaccharide (LPS) treatment first increased Bp LRRm mRNA levels at 3 h, but levels returned to normal at 12 h, then increased and peaked at 24 h. Recombinant Bp LRRm protein bound pathogen-related molecular patterns (PAMPs), including LPS, peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly IC), in a dose-dependent manner. Thus, Bp LRRm might function as a pattern recognition receptor (PRR) in the innate immunity of B. plicatilis, and mediate responses to environmental pollution. Highlights: Molecular characterization of a novel LRR domain containing membrane protein gene ( Bp LRRm) in rotifer Brachionus plicatilis . The mRNA transcripts of Bp LRRm could respond to BDE-47 or LPS stimulation with different profiles. The recombinant protein of Bp LRRm could bind different PAMPs.