Aliidiomarina quisquiliarum sp. nov., isolated from landfill leachate. Issue 2 (7th February 2023)

• Record Type: Journal Article
• Authors: Zhang, Xuan-qi; Xamxidin, Maripat; Li, Jing-hang; Zhang, Xin-yin; Wang, Tao; Chen, Can; Wu, Min; Ke, Han
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 2(2023)
• Publication Date: 2023-02-07
• Subjects: Aliidiomarina -- landfill -- leachate -- draft genome -- phylogenetic analysis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005685
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Bacterial strain Y-6 T, isolated from a landfill site in Yiwu, PR China, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed pale beige colonies. Strain Y-6 T grew at 4–40 °C (optimal at 30–37 °C), pH 6.5–9.5 (optimal at pH 7.2–8.5) and in the presence of 0.5–10.0 % (w/v) NaCl (optimal at 1.0–3.0 %). Phylogenetic analysis revealed that strain Y-6 T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis MCCC 1A06493 T with a 16S rRNA sequence similarity of 98.2 %. The major cellular fatty acids of the isolate were iso-C15 : 0, C16 : 0, iso-C17 : 0 and summed feature 9 (iso-C17 : 1 ω 9 c and/or 10-methyl-C16 : 0 ). Q-8 was the predominant ubiquinone. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycophospholipid, aminophospholipid, phospholipid, three glycolipids and two unknown lipids. The genomic DNA G+C content was 46.6 mol%. The digital DNA–DNA hybridization value between Y-6 T and A. taiwanensis MCCC 1A06493 T was 18.3 %. Strain Y-6 T had an average nucleotide identity value of 74.09 % with A. taiwanensis MCCC 1A06493 T . Results from the polyphasic taxonomy study support the conclusion that strain Y-6 T represents a novel Aliidiomarina species, for which the name Aliidiomarina quisquiliarum sp.nov. is proposed. The type strain is Y-6 T (=MCCC 1K06228 T =KCTC 82676 T ).