A practical approach to producing the single-arm linear dextrin, a chimeric glucosaccharide containing an (α-1 → 4) linked portion at the nonreducing end of an (α-1 → 6) glucochain. (1st April 2023)

• Record Type: Journal Article
• Authors: Wu, Yazhen; Li, Xiaoxiao; Jin, Zhengyu; Svensson, Birte; Bai, Yuxiang
• Is Part Of: Carbohydrate polymers. Volume 305(2023)
• Publication Date: 2023-04-01
• Subjects: CD cyclodextrin -- CLD chain length distribution -- DGase dextran glucosidase -- DP degree of polymerization -- DWS debranched waxy corn starch -- F fructose -- FT-IR Fourier transform infrared spectroscopy -- G glucose -- G2 maltose -- G3 maltotriose -- G4 maltotetraose -- G5 maltopentaose -- G6 maltohexaose -- G7 maltoheptaose -- Gn malto-oligosaccharides of higher degrees of polymerization -- HPAEC-PAD high performance anion exchange chromatograph with pulsed amperometric detection -- HPGPC-RID high performance gel permeation chromatograph with a refractive index detector -- HPLC high performance liquid chromatography -- I2 isomaltose -- I3 isomaltotriose -- IMOs isomalto-oligosaccharides -- In isomalto-oligosaccharides of higher degrees of polymerization -- L leucrose -- LD linear dextrin -- Mn number average molecular weight -- Mw weight mean relative molecular mass -- NMR nuclear magnetic resonance -- PDI polydispersity index -- Res resveratrol -- S sucrose -- SLD single-arm linear dextrin -- XRD X-ray diffraction; Single-arm linear dextrin -- Isomalto-oligosaccharides -- γ-CGTase -- γ-CD -- Chimeric glucosaccharide -- Solubility; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120520
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: How to improve the solubility of linear dextrins (LD) and retain their characteristic helix amphiphilic cavities with flexible embedding capability, is a question worth exploring without adding new chemical groups. The strategy presented in this study is to attach a highly flexible (α-1 → 6) glucochain at the reducing end of LD by preparing a new type of dextrin, referred to as single-arm linear dextrin (SLD). In the actual synthesis, an (α-1 → 6) linked oligosaccharide of DP ¯ 10.7 (PDI = 1.28) was formed by extension of glucose units onto sucrose (2 M) by using L940W mutant of the glucansucrase GTF180-ΔN firstly. Next using γ-CD as glucosylation donor γ-CGTase extended this (α-1 → 6) glucochain with (α-1 → 4) bonds. SLD is a chimeric glucosaccharide comprising an (α-1 → 4) linked part ( DP ¯ 10.5) attached to the nonreducing end of an (α-1 → 6) glucochain as verified by enzyme fingerprinting and 1 H NMR. Furthermore, SLD was validated to show greatly improved solubility and dispersibility of resveratrol in water, as indicated by a 3.12-fold enhancement over the solubility in the presence of 0.014 M SLD. This study provided a new strategy for solving the solubility problem of LD and opens possibilities for new design of the fine structure of starch-like materials. Graphical abstract: Unlabelled Image