Biosystem design of Corynebacterium glutamicum for bioproduction. (February 2023) - Zha, Jian
Abstract : Corynebacterium glutamicum, a natural glutamate-producing bacterium adopted for industrial production of amino acids, has been extensively explored recently for high-level biosynthesis of amino acid derivatives, bulk chemicals such as organic acids and short-chain alcohols, aromatics, and natural products, including polyphenols and terpenoids. Here, we review the recent advances with a focus on biosystem design principles, metabolic characterization and modeling, omics analysis, utilization of nonmodel feedstock, emerging CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) tools for Corynebacterium strain engineering, biosensors, and novel strains of C. glutamicum . Future research directions for developing C. glutamicum cell factories are also discussed. Highlights: Corynebacterium glutamicum has been used for many applications beyond amino acids. Economical, high-quality genome assemblies foster development of non-type strain isolates. Advances and challenges using this host for heterologous bioproductions are discussed. Tool development and modeling deployed in this host enable shorter development cycles.