Characterization and Bioactivities of Sequentially-Prepared Sea Cucumber Ethanolic Extracts and Protein Hydrolysates. Issue 1 (2nd January 2023)

• Record Type: Journal Article
• Authors: Dewi, Ariyanti S.; Arumia, Mei; Samodro, Dohan A.; Fajarningsih, Nurrahmi D.; Patantis, Gintung; Nursid, Muhammad; Batubara, Irmanida; Fawzya, Yusro N.
• Is Part Of: Journal of aquatic food product technology. Volume 32:Issue 1(2023)
• Page Start: 95
• Page End: 110
• Publication Date: 2023-01-02
• Subjects: Sea cucumber -- radical scavenging -- tyrosinase -- glycation; Fishery processing -- Periodicals; Animal products -- Periodicals; Plant products -- Periodicals; 664.9405
• DOI: 10.1080/10498850.2022.2163862
• Languages: English
• ISSNs: 1049-8850

Abstract

ABSTRACT: The sea cucumber ethanol extracts (SCEEs) and protein hydrolysates (SCPHs) of Holothuria atra (HA), H. leucospilota (HL), and Bohadschia marmorata (BM) were prepared sequentially using 60% ethanol and Neutrase. The SCEE of HL contained phenolic acids (4.63 ± 0.50 µg GAE/mg extract) and was the most potent radical scavenger (91.76 ± 0.76%) at 10 mg/mL; whereas its SCPH contained hydrophobic amino acids (31.25 g/100 g) and inhibited glycation by 86.16 ± 5.92% at 5 mg/mL. Furthermore, the SCEE of HA inhibited tyrosinase by 49.93 ± 0.27% at 10 mg/mL, related to sulfated saponins (121.82 ± 3.52 µg xylose/mg extract). This study reports SCEEs as antityrosinase and SCPHs as antiglycation agents for the first time.