Independent roles of the priming and the triggering of the NLRP3 inflammasome in the heart. (18th December 2014)

• Record Type: Journal Article
• Authors: Toldo, Stefano; Mezzaroma, Eleonora; McGeough, Matthew D.; Peña, Carla A.; Marchetti, Carlo; Sonnino, Chiara; Van Tassell, Benjamin W.; Salloum, Fadi N.; Voelkel, Norbert F.; Hoffman, Hal M.; Abbate, Antonio
• Is Part Of: Cardiovascular research. Volume 105:Number 2(2015)
• Page Start: 203
• Page End: 212
• Publication Date: 2014-12-18
• Subjects: NLRP3 -- Inflammasome -- Priming -- Cryopyrinopathy -- Cardiomyopathy -- Caspase-1; Cardiovascular system -- Diseases -- Periodicals; Cardiovascular system -- Periodicals; 616.1
• DOI: 10.1093/cvr/cvu259
• Languages: English
• ISSNs: 0008-6363

Abstract

Abstract: Aims: The NLRP3 inflammasome is activated in the ischaemic heart promoting caspase-1 activation, inflammation, and cell death. Ischaemic injury establishes both a priming signal (transcription of inflammasome components) and a trigger (NLRP3 activation). Whether NLRP3 activation, without priming, induces cardiac dysfunction and/or failure is unknown. The aim of this study was to assess the independent and complementary roles of the priming and the triggering signals in the heart, in the absence of ischaemia or myocardial injury. Methods and results: We used mice with mutant NLRP3 (constitutively active), NLRP3-A350V, under the control of tamoxifen-driven expression of the Cre recombinase ( Nlrp3-A350V/CreT mice). The mice were treated for 10 days with tamoxifen before measuring the activity of caspase-1, the effector enzyme in the inflammasome. Tamoxifen treatment induced the inflammasome in the spleen but not in the heart, despite expression of the mutant NLRP3-A350V. The components of the inflammasome were significantly less expressed in the heart compared with the spleen. Subclinical low-dose lipopolysaccharide (LPS; 2 mg/kg) in Nlrp3-A350V/CreT mice induced the expression of the components of the inflammasome ( priming ), measured using real-time PCR and western blot, leading to the formation of an active inflammasome (caspase-1 activation) in the heart and LV systolic dysfunction while low-dose LPS was insufficient to induce LV systolic dysfunction in wild-type mice (all P < 0.01 for mutant vs. wild-type mice). Conclusion: The signalling pathway governing the inflammasome formation in the heart requires a priming signal in order for an active NLRP3 to induce caspase-1 activation and LV dysfunction.