Construction of vascularized tissue-engineered breast with dual angiogenic and adipogenic micro-tissues. (February 2023)

• Record Type: Journal Article
• Authors: Ni, Ruopiao; Luo, Chao; Ci, Hai; Sun, Di; An, Ran; Wang, Zhenxing; Yang, Jie; Li, Yiqing; Sun, Jiaming
• Is Part Of: Materials today bio. Volume 18(2023)
• Publication Date: 2023-02
• Subjects: Tissue-engineered breast -- Micro-tissue -- Microfluidic technology -- 3D printing -- Breast reconstruction; TEBG Tissue-engineered breast graft -- AM Adipose micro-tissue -- VM Vessel micro-tissue -- ADSC Adipose-derived stem cell -- HUVEC Human umbilical vein endothelial cell -- COL Collagen microgel -- TPU Thermoplastic urethane; Materials science -- Periodicals; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 620.1
• DOI: 10.1016/j.mtbio.2022.100539
• Languages: English
• ISSNs: 2590-0064

Abstract

Abstract: Hydrogel-based micro-tissue engineering technique, a bottom-up approach, is promising in constructing soft tissue of large size with homogeneous spatial distribution and superior regeneration capacity compared to the top-down approach. However, most of the studies employed micro-tissues with simple mesenchymal stem cells, which could hardly meet the growth of matrix and vessels. Therefore, we recommend a dual micro-tissues assembly strategy to construct vascularized tissue-engineered breast grafts (TEBGs). Adipose micro-tissues (AMs) and vessel micro-tissues (VMs) were fabricated by seeding adipose-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs) on collagen microgels (COLs) with a uniform diameter of ∼250 ​μm, respectively. TEBGs were constructed by injecting the dual micro-tissues into 3D printed breast-like Thermoplastic Urethane (TPU) scaffolds, then implanted into the subcutaneous pockets on the back of nude mice. After 3 months of implantation, TEBGs based on dual micro-tissues performed larger volume of adipose tissue regeneration and neo-vessel formation compared to TEBGs based on single AMs. This study extends the application of micro-tissue engineering technique for the construction of soft grafts, and is expected to be useful for creating heterogeneous tissue constructs in the future. Graphical abstract: Assembling dual adipogenic and angiogenic micro-tissues based on ADSCs and HUVECs with 3D printed scaffold is a method of building vascularized tissue-engineered breast. Image 1