Lacticaseibacillus paracasei AO356 ameliorates obesity by regulating adipogenesis and thermogenesis in C57BL/6J male mice. (February 2023)

• Record Type: Journal Article
• Authors: Kim, Young In; Lee, Eun-Sook; Song, Eun-Ji; Shin, Dong-Uk; Eom, Ji-Eun; Shin, Hee Soon; Kim, Jung Eun; Oh, Ju Yeoun; Nam, Young-Do; Lee, So-Young
• Is Part Of: Journal of functional foods. Volume 101(2023)
• Publication Date: 2023-02
• Subjects: Lactobacillus -- Probiotics -- Gutmicrobiota -- Thermogenesis -- Antiobesity; AO356 L. paracasei ssp. tolerans AO356 -- Fabp4 fatty acid binding protein -- BAT brown adipose fat -- BW body weight -- C/ebpα CCAAT/enhancer binding protein α -- Cidea cell death-inducing DNA fragmentation factor-like effector A -- Cpt1 carnitine palmitoyltransferase 1 -- Epi epididymal fat -- Fas fatty acid synthase -- Gas gastrocnemius muscle -- GTE green tea extract -- LPS lipopolysaccharide -- MCP-1 monocyte chemoattractant protein-1 -- ND normal diet -- Pgc1a peroxisome proliferator-activated receptor gamma coactivator 1-alpha -- Pparg peroxisome proliferator-activated gamma -- Retro retroperitoneal -- Prdm16 PR domain containing 16 -- Quad quadriceps muscle -- Srebp1c sterol-regulatory element binding protein-1c -- Sol soleus muscle -- SubQ subcutaneous fat -- TC total cholesterol -- TG triglycerides -- Ucp1 uncoupling protein-1; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2023.105404
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Lacticaseibacillus paracasei AO356 decreased high-fat diet-induced body weight gain, and fat mass. Lacticaseibacillus paracasei AO356 decrease adipocyte size and increase skeletal muscle fiber size in C57BL/6J male mice. Lacticaseibacillus paracasei AO356 downregulated the expression of adipogenesis transcription factors in epididymal and subcutaneous fat pads. Lacticaseibacillus paracasei AO356 upregulated the expression of thermogenesis-related genes in epididymal and subcutaneous fat pads. Abstract: Obesity is a metabolic disease caused by the excessive fat accumulation and is a global health problem that affects more than one-third of the population. Probiotics are considered an effective alternate treatment for preventing obesity as FDA-approved anti-obesity drugs are associated with side effects, such as depression, diarrhea, and dyspepsia. Oral administration of Lacticaseibacillus paracasei AO356 (AO356) isolated from healthy female Koreans to high-fat diet-fed C57BL/6J male mice effectively ameliorated body weight gain, abnormal body composition, and obesity-induced hyperleptinemia and inflammation. This effect may be associated with the downregulation of adipogenesis transcription factor-and lipid metabolism related genes, such as Srebp1c, Pparγ, Fas, C/ebpα, and Fabp4 and upregulation of thermogenesis-related genes, such as Ucp1, Cpt1, Pgc1α, Cidea, and Prdm16 in epididymal and subcutaneous fat pads. In conclusion, our results show that AO356 prevents high-fat diet-induced obesity by regulating adipogenesis and thermogenesis.