Polyphosphate metabolic gene expression analyses reveal mechanisms of phosphorus accumulation and release in Microlunatus phosphovorus strain JN459. Issue 6 (22nd February 2018)

• Record Type: Journal Article
• Authors: Zhong, Chuanqing; Fu, Jiafang; Jiang, Tianyi; Zhang, Chunming; Cao, Guangxiang
• Is Part Of: FEMS microbiology letters. Volume 365:Issue 6(2018:Mar.)
• Publication Date: 2018-02-22
• Subjects: polyphosphate -- phosphorus-accumulating organisms -- polyphosphate glucokinase -- polyphosphate/ATP-dependent NAD kinase; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fny034
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: The ability of Microlunatus phosphovorus to accumulate large amounts of polyphosphate (Poly-P) plays an important role in removing soluble phosphorus from wastewater. Strain JN459, isolated from a sewage system, was previously demonstrated to be Microlunatus phosphovorus. In this study, we analyzed the phosphorus-accumulating and phosphorus-releasing characteristics of strain JN459. Our analyses indicate that strain JN459 accumulates Poly-P under aerobic conditions but releases phosphorus under anaerobic conditions. To determine the mechanisms underlying Poly-P metabolism in strain JN459, we compared transcriptional profiles under aerobic and anaerobic conditions. Significant differences were detected in the expression levels of genes associated with Poly-P metabolism between aerobic and anaerobic conditions, including ppk (MLP_47700, MLP_50300 and MLP_05750), ppgk (MLP_05430 and MLP_26610), ppx (MLP_44770), pap (MLP_23310) and ppnk (MLP_17420). The high expression of polyphosphate glucokinase (MLP_05430) and polyphosphate/ATP-dependent NAD kinase (MLP_17420) indicated that both of them might be responsible for utilizing Poly-P as the energy resource for growth under anaerobic conditions. These findings enhance our understanding of phosphate metabolism in a major bacterial species involved in wastewater phosphorus reduction. Abstract : Transcriptional profiles under aerobic and anaerobic conditions were compared to determine the mechanisms underlying polyphosphate metabolism in Microlunatus phosphovorus .