DNA-decorated multilamellar cholesterol assemblies for nucleic acid detection in the micrometer-scale solid-state nanopore. (13th February 2023)

• Record Type: Journal Article
• Authors: Ye, Jianhan; Lu, Xin; Zhao, Xiaohuan; Wu, Fen; Dong, Yuanchen; Shi, Qian; Xu, Lijin
• Is Part Of: Polymer. Volume 267(2023)
• Publication Date: 2023-02-13
• Subjects: DNA -- Cholesterol -- Assembly -- Nanopore -- Nucleic acid detection; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2023.125676
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: The large-sized glass nanopipette (GN) allows for an access of large targets and show the benefits of excellent mechanical stability and reproducibility. The nanoscale DNA-decorated multilamellar cholesterol assemblies are introduced into the GN with several nanometers, and could in situ grow into a micrometer-scale aggregates under DNA signal stimulus, developing a simple and sensitive strategy for DNA detection in the micrometer-scale GN. The detection limit of target DNA reaches 2.69 fM in a GN with a diameter of up to ca. 4.76 μm. Graphical abstract: We explore the possibility of using DNA-amphiphile assemblies as probe carriers for DNA detection in conjunction with micrometer-scale solid-state nanopore. Image 1 Highlights: A novel DNA-dendron hybrid was designed and synthesized. The nanoscale multilamellar assemblies were transformed to micrometer-scale aggregates in response to DNA signal. DNA-amphiphile supramolecular assemblies were first in combination with glass micropipettes for nucleic acids detection. The LOD of nucleic acids could be 2.69 fM.