Biological autoluminescence as a perturbance-free method for monitoring oxidation in biosystems. (January 2023)

• Record Type: Journal Article
• Authors: Vahalová, Petra; Cifra, Michal
• Is Part Of: Progress in biophysics and molecular biology. Volume 177(2023)
• Page Start: 80
• Page End: 108
• Publication Date: 2023-01
• Subjects: Biological luminescence -- Oxidative stress -- Monitoring methods; Biophysics -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular Biology -- Periodicals; Biophysique -- Périodiques; Biochimie -- Périodiques; 571.4
• DOI: 10.1016/j.pbiomolbio.2022.10.009
• Languages: English
• ISSNs: 0079-6107

Abstract

Abstract: Biological oxidation processes are in the core of life energetics, play an important role in cellular biophysics, physiological cell signaling or cellular pathophysiology. Understanding of biooxidation processes is also crucial for biotechnological applications. Therefore, a plethora of methods has been developed for monitoring oxidation so far, each with distinct advantages and disadvantages. We review here the available methods for monitoring oxidation and their basic characteristics and capabilities. Then we focus on a unique method — the only one that does not require input of additional external energy or chemicals — which employs detection of biological autoluminescence (BAL). We highlight the pros and cons of this method and provide an overview of how BAL can be used to report on various aspects of cellular oxidation processes starting from oxygen consumption to the generation of oxidation products such as carbonyls. This review highlights the application potential of this completely non-invasive and label-free biophotonic diagnostic method.