Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes. (1st September 2021)

• Record Type: Journal Article
• Authors: Ouattara, Mahamoudou; Tamboura, Mamadou; Kambiré, Dinanibè; Lê, Kim Anh; Van Phan, Thanh; Velusamy, Srinivasan; Nguyen, Hien Anh; Trang, Dai Vo Thi; Lessa, Fernanda C; Iijima, Makiko; Nguyen, Dac Trung; Schwartz, Stephanie B; McGee, Lesley; Traoré, Rasmata Ouédraogo; Beall, Bernard
• Is Part Of: Journal of infectious diseases. Volume 224:Supplement 3(2021)
• Page Start: S204
• Page End: S208
• Publication Date: 2021-09-01
• Subjects: Streptococcus pneumoniae -- direct real-time PCR -- pneumococcal serotype; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiab056
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.