The Search for a Genetic Factor Associating with Immune Restoration Disease in HIV Patients Co-Infected with Mycobacterium tuberculosis. Issue 6 (21st May 2013)

• Record Type: Journal Article
• Authors: Affandi, Jacquita S.; Kumar, Manoj; Agarwal, Upasna; Singh, Sarman; Price, Patricia
• Is Part Of: Disease markers. Volume 34:Issue 6(2013)
• Page Start: 445
• Page End: 449
• Publication Date: 2013-05-21
• Subjects: TB-IRD susceptibility -- immunogenetic factor -- single nucleotide polymorphism; Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.3233/DMA-130991
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : BACKGROUND: Up to 43% of HIV-infected patients co-infected with Mycobacterium tuberculosis experience exacerbations of tuberculosis (TB) after commencing antiretroviral therapy (ART). These are termed immune restoration disease (IRD). It is unclear why individual susceptibility varies. OBJECTIVE: We investigate if single nucleotide polymorphisms (SNP) in genes encoding cytokines, chemokines and their receptors associate with development of an IRD event in patients of two different ethnicities. METHODS: DNA samples were available from small well-characterised groups of HIV patients treated in Cambodia (TB-IRD, n = 17; HIV + TB + controls, n = 55) and India (TB-IRD, n = 19; HIV + TB + controls, n = 43). HIV patients with a TB diagnosis but no evidence of IRD were included to control for susceptibility to TB per se. Sixteen SNP implicated in inflammation or mycobacterial disease were genotyped. RESULTS: Susceptibility to TB-IRD associated with carriage of TNFA-1031*T (rs1799964; P =0.05) and SLC11A1 D543N*G (rs17235409; P =0.04) in Cambodian patients and carriage of IL18-607*G (rs1946518; P =0.02) and VDR FokI (F/f)*T (rs10735810; P =0.05) in Indian patients. CONCLUSIONS: Associations between polymorphisms in immune-related genes and TB-IRD were found, but none were common across two ethnicities.