Hsa_circ_0009910: oncogenic circular RNA targets microRNA-145 in ovarian cancer cells. Issue 15 (2nd August 2020)

• Record Type: Journal Article
• Authors: Li, Ying; Lin, Shuang; An, Na
• Is Part Of: Cell cycle. Volume 19:Issue 15(2020)
• Page Start: 1857
• Page End: 1868
• Publication Date: 2020-08-02
• Subjects: Hsa_circ_0009910 -- microrna-145 -- ovarian cancer -- proliferation -- motility; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2020.1731650
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Circular RNAs (circRNAs) correlate with cancer cell phenotypes. Particularly, circRNAs mediate the cancer process as microRNAs (miRNAs) sponges. This study was to ascertain the roles of hsa_circ_0009910 in phenotypic aspects of ovarian cancer cells. Mantel-Cox test was performed to analyze the correlation between hsa_circ_0009910 and survival outcomes of ovarian cancer. Minigene reporter was constructed and small interfering-RNA was designed for constructing hsa_circ_0009910-dysregulated and miR-145-upregulated cells identified by qRT-PCR. Proliferative and motile activities were monitored by CCK and Transwell. Western blot was applied for quantification of cyclin D1, CDK4, CDK6, MMP-2, MMP-9, IκBα, p65, Notch1, Hes1, and Hes5. miRNAs targets were predicted using a bioinformatics tool and confirmed using qRT-PCR and Dual-Luciferase reporter assay. Hsa_circ_0009910 was correlated with the poor prognosis of ovarian cancer patients. The ovarian cancer cell phenotypes were promoted by hsa_circ_0009910 while repressed by silencing hsa_circ_0009910. Hsa_circ_0009910 silence was responsible for the upregulation of the predicted miRNAs targets. Thereinto, miR-145 was confirmed as a miRNA target and negatively regulated by hsa_circ_0009910. miR-145 nullified the biological function of hsa_circ_0009910 in the proliferative and motile phenotypes, and the active status of NF-κB and Notch. Hsa_circ_0009910, representing unfavorable prognosis, induced proliferative and motile phenotypes by suppressing miR-145 in ovarian cancer cells.