Sequencing, assembly and annotation of the whole-insect genome of Lymantria dispar dispar, the European gypsy moth. Issue 8 (30th April 2021)

• Record Type: Journal Article
• Authors: Sparks, Michael E; Hebert, Francois Olivier; Johnston, J Spencer; Hamelin, Richard C; Cusson, Michel; Levesque, Roger C; Gundersen-Rindal, Dawn E
• Editors: Oliver, B
• Is Part Of: G3. Volume 11:Issue 8(2021)
• Publication Date: 2021-04-30
• Subjects: whole-genome sequencing -- PacBio long-read assembly -- Illumina short-read polishing -- automated gene finding -- Lepidoptera -- European gypsy moth -- gypsy moth genomics; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1093/g3journal/jkab150
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: The European gypsy moth, Lymantria dispar dispar (LDD), is an invasive insect and a threat to urban trees, forests and forest-related industries in North America. For use as a comparator with a previously published genome based on the LD652 pupal ovary-derived cell line, as well as whole-insect genome sequences obtained from the Asian gypsy moth subspecies L. dispar asiatica and L. dispar japonica, the whole-insect LDD genome was sequenced, assembled and annotated. The resulting assembly was 998 Mb in size, with a contig N50 of 662 Kb and a GC content of 38.8%. Long interspersed nuclear elements constitute 25.4% of the whole-insect genome, and a total of 11, 901 genes predicted by automated gene finding encoded proteins exhibiting homology with reference sequences in the NCBI NR and/or UniProtKB databases at the most stringent similarity cutoff level (i.e., the gold tier). These results will be especially useful in developing a better understanding of the biology and population genetics of L. dispar and the genetic features underlying Lepidoptera in general.