The Neurospora Transcription Factor ADV-1 Transduces Light Signals and Temporal Information to Control Rhythmic Expression of Genes Involved in Cell Fusion. Issue 1 (1st January 2017)

• Record Type: Journal Article
• Authors: Dekhang, Rigzin; Wu, Cheng; Smith, Kristina M; Lamb, Teresa M; Peterson, Matthew; Bredeweg, Erin L; Ibarra, Oneida; Emerson, Jillian M; Karunarathna, Nirmala; Lyubetskaya, Anna; Azizi, Elham; Hurley, Jennifer M; Dunlap, Jay C; Galagan, James E; Freitag, Michael; Sachs, Matthew S; Bell-Pedersen, Deborah
• Is Part Of: G3. Volume 7:Issue 1(2017)
• Page Start: 129
• Page End: 142
• Publication Date: 2017-01-01
• Subjects: phototransduction -- circadian clock -- development -- regulatory network; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.116.034298
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Light and the circadian clock have a profound effect on the biology of organisms through the regulation of large sets of genes. Toward understanding how light and the circadian clock regulate gene expression, we used genome-wide approaches to identify the direct and indirect targets of the light-responsive and clock-controlled transcription factor ADV-1 in Neurospora crassa . A large proportion of ADV-1 targets were found to be light- and/or clock-controlled, and enriched for genes involved in development, metabolism, cell growth, and cell fusion. We show that ADV-1 is necessary for transducing light and/or temporal information to its immediate downstream targets, including controlling rhythms in genes critical to somatic cell fusion. However, while ADV-1 targets are altered in predictable ways in Δ adv-1 cells in response to light, this is not always the case for rhythmic target gene expression. These data suggest that a complex regulatory network downstream of ADV-1 functions to generate distinct temporal dynamics of target gene expression relative to the central clock mechanism.