Environmentally relevant concentration of sulfamethoxazole-induced oxidative stress-cascaded damages in the intestine of grass carp and the therapeutic application of exogenous lycopene. (1st April 2021)

• Record Type: Journal Article
• Authors: Wang, Yu; Zhao, Hongjing; Liu, Yachen; Li, Jingyan; Nie, Xiaopan; Huang, Puyi; Xing, Mingwei
• Is Part Of: Environmental pollution. Volume 274(2021)
• Publication Date: 2021-04-01
• Subjects: Antibiotic -- Oxidative stress -- Inflammation -- Apoptosis -- Autophagy -- Antioxidant; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.116597
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Due to the unreasonable use and discharge of the aquaculture industry, over standard of the antibiotics has been frequent in different types of water environments, causing adverse effects on aquatic organisms. Lycopene (LYC) is an esculent carotenoid, which is considered to be a strong antioxidant. This study was designed to explore the therapeutic effect of LYC on antibiotic (sulfamethoxazole (SMZ)) induced intestinal injury in grass carp Ctenopharyngodon idella . The 120 carps (the control, LYC, SMZ, and co-administration groups) were treated for 30 days. We found that treatment with LYC significantly suppressed SMZ-induced intestinal epithelial cell damage and tight junction protein destruction through histopathological observation, transmission electron microscopy and detection of related genes (Claudin-1/3/4, Occludin and zonula occludens (ZO)-1/2). Furthermore, LYC mitigated SMZ-induced dysregulation of oxidative stress markers, including elevated malondialdehyde (MDA) levels, and consumed super oxide dimutese (SOD), catalase (CAT) activities and glutathione (GSH) content. In the same treatment, LYC reduced inflammation and apoptosis by a detectable change in pro-inflammatory factors (tumor necrosis factor-alpha (TNF-β), interleukin (IL)-1β, IL-6 and IL-8), anti-inflammatory factors (transforming growth factor-beta (TGF-β) and IL-10) and pro-apoptosis related genes (p53, p53 upregulated modulator of apoptosis (PUMA), Bax/Bcl-2 ratio, caspase-3/9). In addition, activation of autophagy (as indicated by increased autophagy-related genes through AMPK/ATK/MTOR signaling pathway) under the stress of SMZ was also dropped back to the original levels by LYC co-administration. Collectively, our findings identified that LYC can serve as a protectant agent against SMZ-induced intestinal injury. Graphical abstract: Image 1 Highlights: Sulfamethoxazole induces intestinal damage and tight junction protein destruction. Sulfamethoxazole induces oxidative stress, inflammation and apoptosis in intestine. Lycopene mitigates sulfamethoxazole-induced toxicity via AMPK/ATK/MTOR pathway. Abstract : Sulfamethoxazole led to intestinal oxidative stress and inflammatory lesions, which were relieved by exogenous lycopene co-administration via the activation of Akt/mTOR signaling pathway.