Time‐resolved microscopy made easy: New confocal microscope for dynamic structural biology with single‐molecule FRET. Issue 1 (3rd January 2023)

• Record Type: Journal Article
• Authors: Loidolt‐Krüger, Maria
• Is Part Of: PhotonicsViews. Volume 20:Issue 1(2023)
• Page Start: 40
• Page End: 44
• Publication Date: 2023-01-03
• Subjects: Photonics -- Periodicals; Optics -- Periodicals; Lasers -- Periodicals; 621.36; 621.36
• DOI: 10.1002/phvs.202300002
• Languages: English
• ISSNs: 2626-1294

Abstract

Abstract: Quantitative single molecule and time‐resolved fluorescence techniques offer new insights into many samples in life and materials sciences. So far, their adoption has been slow because expert knowledge was required for correct data acquisition and analysis. Now, PicoQuant have developed a new confocal microscope called Luminosa. It combines state‐of‐the‐art hardware with cutting‐edge software to deliver high‐quality data while simplifying daily operation. The software includes context‐based workflows for each technique. This article shows how it streamlines single‐molecule Förster resonance energy transfer (smFRET) experiments.