Expression of netrin and its receptors uncoordinated‐5 and frazzled in arthropods and onychophorans suggests conserved and diverged functions in neuronal pathfinding and synaptogenesis. Issue 1 (15th February 2022)

• Record Type: Journal Article
• Authors: Janssen, Ralf; Budd, Graham E.
• Other Names: Deans Michael R. guestEditor.; Williams Megan E. guestEditor.; Kale Shubham guestEditor.
• Is Part Of: Developmental dynamics. Volume 252:Issue 1(2023)
• Page Start: 172
• Page End: 185
• Publication Date: 2022-02-15
• Subjects: Arthropoda -- frazzled -- nervous system development -- netrin -- onychophora -- Panarthropoda -- Uncoordinated‐5; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.459
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: Development of the nervous system and the correct connection of nerve cells require coordinated axonal pathfinding through an extracellular matrix. Outgrowing axons exhibit directional growth toward or away from external guidance cues such as Netrin. Guidance cues can be detected by growth cones that are located at the end of growing axons through membrane‐bound receptors such as Uncoordianted‐5 and Frazzled. Binding of Netrin causes reformation of the cytoskeleton and growth of the axon toward (or away from) the source of Netrin production. Results: Here, we investigate the embryonic mRNA expression patterns of n etrin genes and their potential receptors, u ncoordinated‐5 and f razzled in arthropod species that cover all main branches of Arthropoda, that is, Pancrustacea, Myriapoda, and Chelicerata. We also studied the expression patterns in a closely related outgroup species, the onychophoran Euperipatoides kanangrensis, and provide data on expression profiles of these genes in larval tissues of the fly Drosophila melanogaster including the brain and the imaginal disks. Conclusion: Our data reveal conserved and diverged aspects of neuronal guidance in Drosophila with respect to the other investigated species and suggest a conserved function in nervous system patterning of the developing appendages. Key Findings: Comprehensive expression analysis of neuronal guidance cues and their receptors. Conserved and diverged aspects of neuronal guidance in panarthropods. Neuronal guidance cues in developing arthropod and onychophoran appendages.