Gamma-oryzanol as a potential modulator of oxidative stress and inflammation via PPAR-y in adipose tissue: a hypothetical therapeutic for cytokine storm in COVID-19?. (15th January 2021)

• Record Type: Journal Article
• Authors: Francisqueti-Ferron, Fabiane Valentini; Garcia, Jéssica Leite; Ferron, Artur Junio Togneri; Nakandakare- Maia, Erika Tiemi; Gregolin, Cristina Schmitt; Silva, Janaina Paixão das Chagas; dos Santos, Klinsmann Carolo; Lo, Ângelo Thompson Colombo; Siqueira, Juliana Silva; de Mattei, Letícia; de Paula, Bruno Henrique; Sarzi, Felipe; Silva, Carol Cristina Vágula de Almeida; Moreto, Fernando; Costa, Mariane Róvero; Ferreira, Ana Lucia A.; Minatel, Igor Otávio; Corrêa, Camila Renata
• Is Part Of: Molecular and cellular endocrinology. Volume 520(2021)
• Publication Date: 2021-01-15
• Subjects: Obesity -- COVID-19 -- Gamma-oryzanol -- PPAR-γ; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2020.111095
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: The literature has reported a higher prevalence of negative clinical outcomes due to Coronavirus disease 19 (COVID-19) in obese individuals. This can be explained by the cytokine storm, result from the cytokine production from both obesity and viral infection. Gamma-oryzanol (γOz) is a compound with anti-inflammatory and antioxidant activities. However, little is known about the γOz action as a possible agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). The aim of this study was to test the hypothesis that γOz attenuates the cytokine storm by stimulating PPAR-γ in the adipose tissue. Methods : Male Wistar rats were randomly divided into three experimental groups and fed ad libitum for 30 weeks with control diet (C, n = 6), high sugar-fat diet (HSF, n = 6) or high sugar-fat diet + γOz (HSF + γOz, n = 6). HSF groups also received water + sucrose (25%). The γOz dose was 0.5% in the chow. Evaluation in animals included caloric intake, body weight, adiposity index, plasma triglycerides, and HOMA-IR. In adipose tissue was evaluated: PPAR-γ gene and protein expression, inflammatory and oxidative stress parameters, and histological analysis. Results: Adipose tissue dysfunction was observed in HSF group, which presented remarkable PPAR-γ underexpression and increased levels of cytokines, other inflammatory markers and oxidative stress. The γOz treatment prevented adipose tissue dysfunction and promoted PPAR-γ overexpression. Conclusion: Natural compounds as γOz can be considered a coadjutant therapy to prevent the cytokine storm in COVID-19 patients with obesity conditions. Highlights: Gamma oryzanol is a compound of rice bran with anti-inflammatory and antioxidant action in adipose tissue. Insulin resistance, dyslipidemia and cardiac dysfunction were attenuated by gamma oryzanol. PPAR-gamma increases its expression with gamma oryzanol in adipose tissue. Gamma oryzanol may be an alternative compound in the treatment of cytokine storm in obese individuals with COVID-19.