Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. (October 2015)

• Record Type: Journal Article
• Authors: Grujić, Marica; Dojnov, Biljana; Potočnik, Ivana; Duduk, Bojan; Vujčić, Zoran
• Is Part Of: International biodeterioration & biodegradation. Volume 104(2015)
• Page Start: 290
• Page End: 298
• Publication Date: 2015-10
• Subjects: Spent mushroom compost -- Trichoderma -- Amylase -- Cellulase -- Xylanase; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2015.04.029
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and β-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer ( Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL −1 ), five isoforms of β-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL −1 ) and xylanase (2.31 U mL −1 ). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates. Highlights: SMC volume was reduced by 30% after the production of fungal maintains and enzyme. SMC proved to be a good substrate for fungal production of cellulolytic enzymes. Trichoderma spp. utilizes SMC waste more efficient than reference isolate Aspergillus niger. Trichoderma atroviride (T42) represents a new potential wt. strain for industrial purposes.