High throughput screening of enzyme activity with mass spectrometry imaging. (February 2015)

• Record Type: Journal Article
• Authors: de Rond, Tristan; Danielewicz, Megan; Northen, Trent
• Is Part Of: Current opinion in biotechnology. Volume 31(2015)
• Page Start: 1
• Page End: 9
• Publication Date: 2015-02
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2014.07.008
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Mass spectrometry is a versatile alternative to colorimetric enzyme activity assays. Technologies developed for MSI can be applied to high-throughput enzyme screening. Analyte immobilization allows for activity determination in complex sample matrices. Such methods along with selective derivatization reactions enable label-free assays. These advances could aid the spatial localization of enzymatic activity with MSI. Abstract : Mass spectrometry imaging (MSI) has found a diversity of applications ranging from localizing metabolites and proteins in tissues to investigating microbial interactions, and as a result is perhaps the fastest growing subfield of mass spectrometry. Advances in surface mass spectrometry technologies are equally applicable to the analysis of arrayed samples. One promising field in which this capacity has been leveraged is the high-throughput analysis of enzyme activity, an important step in the development of a wide range of biotechnologies. This review article describes several emerging approaches that seek to improve the quality and scope of this application of MSI.