(Invited) Observation of Stress Responses of Bacteria Confined in a MEMS Microfluidic Chip. (14th September 2015)

• Record Type: Journal Article
• Authors: Nishimura, Yusuke; Ishida, Makoto; Sawada, Kazuaki; Ishii, Hiromu; Machida, Katsuyuki; Masu, Kazuya; Wang, Changle; Iida, Kenichiro; Saito, Mitsumasa; Yoshida, Shinichi
• Is Part Of: ECS transactions. Volume 69:Number 10(2015)
• Page Start: 259
• Page End: 267
• Publication Date: 2015-09-14
• Subjects: Electrochemistry -- Periodicals; Electrochemistry; Periodicals; Electronic journals; Electronic journal; 541.37
• DOI: 10.1149/06910.0259ecst
• Languages: English
• ISSNs: 1938-5862

Abstract

Abstract : This work describes the new and simple method for observing bacterial behavior of stress response such as to light irradiation in a polydimethylsiloxane (PDMS) -based MEMS microfluidic chip. The fluorescence was observed from Legionella cells confined in small spaces among microbeads. Ultraviolet (UV) light irradiation was found to be a trigger for producing fluorescent material. Confining bacteria in the small spaces made by the MEMS technique is an effective way for investigating their stress response.