A novel Gynostemma pentaphyllum saponin and its adipogenesis inhibitory effect through modulating Wnt/β-catenin pathway and cell cycle in mitotic clonal expansion. (August 2015)

• Record Type: Journal Article
• Authors: Liu, Jie; Yang, Puyu; Shi, Haiming; Sun, Xiangjun; Lee, Seong-Ho; Yu, Liangli (Lucy)
• Is Part Of: Journal of functional foods. Volume 17(2015)
• Page Start: 552
• Page End: 562
• Publication Date: 2015-08
• Subjects: aP2 adipocyte protein 2 -- C/EBPα CCAAT/enhancer-binding protein α -- C/EBPβ CCAAT enhancer-binding protein β -- C/EBPδ CCAAT/enhancer-binding protein δ -- CHOP-10 C/EBP homologous protein -- DVL disheveled -- ERK extracellular signal-regulated kinase -- FBS fetal bovine serum -- FZD Frizzled -- GSK3β glycogen synthase kinase 3-β -- HPLC high performance liquid chromatography -- MAPK mitogen-activated protein kinase -- MCE mitotic clonal expansion -- MS metabolic syndrome -- NOESY Nuclear Overhauser Enhancement SpectroscopY -- PPARγ peroxisome proliferator-activated receptor γ -- TG triglyceride -- TLC thin layer chromatography; Gynostemma pentaphyllum -- Saponin -- Adipogenesis -- Mitosis clone expansion -- CCAAT/enhancer-binding protein β -- Wnt/β-catenin signaling; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.06.014
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A novel saponin (JS) isolated from tetraploid Gynostemma pentaphyllum was first characterized for its chemical structure and investigated for its inhibitory effect on adipocyte differentiation and molecular mechanisms. JS suppressed 3T3-L1 pre-adipocyte differentiation via inhibiting mitosis clone expansion (MCE), decreasing the DNA-binding activity of C/EBPβ. JS suppressed 3T3-L1 pre-adipocyte differentiation though modulating Wnt/β- catenin pathway. The results from this study may be used to promote the value-added utilization of G. pentaphyllum and the use of its extracts or components as a dietary supplement has the potential as a nutraceutical to prevent the risk of overweight and obesity, without any side effects. Abstract: A novel saponin (JS) was isolated from the tetraploid jiaogulan, a popular tea also known as Gynostemma pentaphyllum, and characterized for its chemical structure. JS was investigated for its inhibitory effect on adipocyte differentiation and the potential molecular mechanisms using 3T3-L1 murine pre-adipocytes. JS treatment down-regulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) at both mRNA and protein levels. Further analysis proved that the inhibition was primarily limited to the early stage of adipocyte differentiation, where JS decreased the DNA-binding activity of C/EBPβ by blocking its phosphorylation. In addition, JS increased phosphorylation of glycogen synthase kinase 3β (GSK-3β) and subsequently increased stability and nuclear translocation of β-catenin through modulating expression of Wnt/β-catenin signaling components. Moreover, JS-induced down regulation of adipogenic markers and fat accumulation is β-catenin-dependent. These data suggested that this novel saponin compound might inhibit adipogenesis through activating Wnt/β-catenin signaling during adipocyte differentiation.