Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo. (1st March 2021)

• Record Type: Journal Article
• Authors: Zhao, Yun-Li; Pu, Shi-Biao; Qi, Yan; Wu, Bai-Fen; Shang, Jian-Hua; Liu, Ya-Ping; Hu, Di; Luo, Xiao-Dong
• Is Part Of: Journal of ethnopharmacology. Volume 267(2021)
• Publication Date: 2021-03-01
• Subjects: Alstonia scholaris -- Indole alkaloids -- Pulmonary fibrosis -- Picrinine -- Vallesamine -- Scholaricine -- 19-Epischolaricine; PF pulmonary fibrosis -- TA total alkaloids -- i.g. intragastric -- Pic picrinine -- Val vallesamine -- Sch scholaricine -- Epi 19-epischolaricine -- TA-H total alkaloids (i.g.), 50 mg/kg -- TA-M total alkaloids (i.g.) 25 mg/kg -- TA-L total alkaloids (i.g.) 12.5 mg/kg -- BLM bleomycin -- PRE prednisone acetate -- MDA malondialdehyde -- SOD superoxide dismutase -- KL-6 krebs. von den lungen-6 -- Hyp hydroxyproline -- Col-1 collagen Ⅰ -- HE hematoxylin and eosin -- TGF-β transforming growth factor beta -- LDH lactate dehydrogenase -- MMP-1 matrix metalloproteinase-1 -- TIMP-1 tissue inhibitor of metalloproteinase-1; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113506
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Alstonia scholaris (L.) R. Br. (Apocynaceae) is a Dai folk medicine for the treatment of lung diseases in China. Aim of the study : The present study investigated the anti-pulmonary fibrosis effects of total alkaloids (TA) and the potential active ingredients and its possible mechanism. Materials and methods: After intratracheal instillation of bleomycin (BLM, 5 mg/kg), mice were divided into ten groups, and orally treated with the corresponding samples once daily for 28 days. The effect of indole alkaloids was determined through analysis of cytokines, as well as histopathological examinations and gene expressions. Results: Severe lung fibrosis was observed in the BLM-treated mice on day 28. However, the administration of TA significantly ameliorated the pathological changes in the lungs, decreased the content of Krebs von den Lungen-6, lactate dehydrogenase, transforming growth factor-β (TGF-β), hydroxyproline, type I collagen, and malonaldehyde, and enhanced the activity of superoxide dismutase in the serum and lung tissues. In addition, the enhanced TGF-β and matrix metalloproteinase-1 (MMP-1) expressions in BLM-induced mice were obviously weakened by indole alkaloids, as well as the ratio of matrix metalloproteinase-1 to tissue inhibitor of metalloproteinase-1 was decreased. Moreover, picrinine and scholaricine yielded markedly better values in the aforementioned indices than those in other samples, indicating that they may be the active ingredients of alkaloids. Conclusions: TA exerted protective effects against BLM-induced pulmonary fibrosis by reducing collagen deposition through TGF-β/MMP-1 pathway. Graphical abstract: Image 1