Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-κB activation and upregulating HO-1 expression. (1st March 2021)

• Record Type: Journal Article
• Authors: Kim, Seong-Man; Ryu, Hyung Won; Kwon, Ok-Kyoung; Hwang, Daseul; Kim, Min Gu; Min, Jae-Hong; Zhang, Zhiyun; Kim, Soo-Yong; Paik, Jin-Hyub; Oh, Sei-Ryang; Ahn, Kyung-Seop; Lee, Jae-Won
• Is Part Of: Journal of ethnopharmacology. Volume 267(2021)
• Publication Date: 2021-03-01
• Subjects: Allergic asthma -- Callicarpa japonica Thunb. -- Th2 cytokines -- Eosinophil -- NF-κB -- HO-1; OVA Ovalbumin IL-4, 5, 6, 8, 13 interleukin-4, 5, 6, 8, 13 -- IgE Immunoglobulin E BALF, bronchoalveolar lavage fluid -- AHR Airway hyperresponsiveness -- CREB cAMP-response element binding protein -- iNOS inducible nitric oxide synthase -- MCP-1 monocyte chemoattractant protein-1 -- NF-κB nuclear factor-κB -- HO-1 heme oxygenase-1; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113523
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Callicarpa japonica Thunb., as an herbal medicine has been used for the treatment of inflammatory diseases in China and Korea. Materials and methods: Ultra performance liquid chromatography–photodiode array–quadrupole time–of–flight mass spectrometer (UPLC–PDA–QTof MS) was used to detect the major phenylethanoid glycosides in the C. japonica extract. BALB/c mice were intraperitoneally sensitized by ovalbumin (OVA) (on days 0 and 7) and challenged by OVA aerosol (on days 11–13) to induce airway inflammatory response. The mice were also administered with C. japonica Thunb. (CJT) (20 and 40 mg/kg Per oral) on days 9–13. CJT pretreatment was conducted in lipopolysaccharide (LPS)-stimulated RAW264.7 or phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells. Results: CJT administration significantly reduced the secretion of Th2 cytokines, TNF-α, IL-6, immunoglobulin E (IgE) and histamine, and the recruitment of eosinophils in an OVA-exposed mice. In histological analyses, the amelioration of inflammatory cell influx and mucus secretion were observed with CJT. The OVA-induced airway hyperresponsiveness (AHR), iNOS expression and NF-κB activation were effectively suppressed by CJT administration. In addition, CJT led to the upregulation of HO-1 expression. In an in vitro study, CJT pretreatment suppressed the LPS-induced TNF-α secretion in RAW264.7 cells and attenuated the PMA-induced IL-6, IL-8 and MCP-1 secretion in A549 cells. These effects were accompanied by downregulated NF-κB phosphorylation and by upregulated HO-1 expression. Conclusion: These results suggested that CJT has protective activity against OVA-induced airway inflammation via downregulation of NF-κB activation and upregulation of HO-1, suggesting that CJT has preventive potential for the development of allergic asthma. Graphical abstract: Image 1