P10 Interactions of fibroblasts derived from irradiated subcutaneous connective tissue with HNSCC. Issue 5 (May 2015)

• Record Type: Journal Article
• Authors: Gehrke, T.; Hackenberg, S.; Scherzad, A.; Kleinsasser, N.
• Is Part Of: Oral oncology. Volume 51:Issue 5(2015:May)
• Page Start: e45
• Publication Date: 2015-05
• Subjects: Mouth -- Cancer -- Periodicals; Mouth -- Tumors -- Periodicals; Mouth Diseases -- Periodicals; Mouth Neoplasms -- Periodicals; Bouche -- Cancer -- Périodiques; Bouche -- Tumeurs -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9943105
• DOI: 10.1016/j.oraloncology.2015.02.058
• Languages: English
• ISSNs: 1368-8375

Abstract

Abstract : Introduction: Cancer cells show a variety of interactions with cells of the surrounding connective tissue, mainly consisting of fibroblasts. Human skin-fibroblasts which are co-cultivated with tumor cells have the ability to convert into carcinoma-associated fibroblasts (CAF's) which are known to support tumor growth and invasiveness. The aim of the current study was to determine the impact of irradiation on fibroblast-tumor interaction. Material: Primary human fibroblasts of subcutaneous connective tissue of pre-irradiated or non-irradiated skin were co-cultivated with the HNSCC cell line FaDu for 3 days. MTT assay, annexin V-propidium iodide test and ELISA were performed to analyze tumor viability, apoptosis and secretion of interleukin (IL)-6 and IL-8. Markers for epithelial-mesenchymal transition (EMT) were assessed by western blot. Results: Co-culture with pre-irradiated fibroblasts showed a significant decrease in tumor viability and an increase in apoptosis. Levels of IL-8 significantly decreased in irradiated compared to non-irradiated fibroblasts. After irradiation, the cells did not promote EMT anymore. Conclusion: Previous radiation influences fundamental properties of the complex tumor and stroma orchestration. These interactions have to be evaluated in detail in order to further illuminate possible options of intervention.