The hydrogel of whey protein isolate coated by lotus root amylopectin enhance the stability and bioavailability of quercetin. (15th May 2020)

• Record Type: Journal Article
• Authors: Liu, Kang; Zha, Xue-Qiang; Shen, Wen-Di; Li, Qiang-Ming; Pan, Li-Hua; Luo, Jian-Ping
• Is Part Of: Carbohydrate polymers. Volume 236(2020)
• Publication Date: 2020-05-15
• Subjects: Lotus rootamylopectin -- Whey protein isolate -- Hydrogels -- Quercetin -- Encapsulation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116009
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: LRA and WPI represents lotus root amylopectin and whey protein isolate, respectively. LRA-coated WPI hydrogel was developed to protect quercetin (QUE). WPI-QUE-LRA particles exhibited a core-shell structure. The stability of quercetin was enhanced by the LRA-coated WPI hydrogel. LRA-coated WPI hydrogel improved the bioavailability of quercetin in mice. Abstract: In this study, whey protein isolate (WPI)-quercetin (Que)-lotus root amylopectin (LRA) hydrogels (WPI-QUE-LRA) was developed to improve the solubility, stability and bioavailability of quercetin. Results showed that the favorable WPI-QUE-LRA was formed using WPI and LRA in the ratio of 1:2 at pH 7.0. Under this condition, the average size, polydispersity index, zeta potential of the WPI-QUE-LRA was 179.5 nm, 0.271, −18.6 mV, respectively. The analysis of transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffractometer revealed that the quercetin was successfully encapsulated in WPI-LRA, giving a high encapsulation efficiency of 92.4 %. Moreover, the WPI-LRA could significantly improve the storage stability and photochemical stability of quercetin. The in vitro and in vivo experiments showed that LRA-coated WPI hydrogel can enable quercetin to be stable in stomach and be effectively released in small intestine, leading to the enhancement of the bioavailability of quercetin.