Chlorogenic acid abates male reproductive dysfunction in arsenic-exposed mice via attenuation of testicular oxido-inflammatory stress and apoptotic responses. (5th January 2021)

• Record Type: Journal Article
• Authors: El-Khadragy, Manal F.; AL-Megrin, Wafa A.; Alomar, Suliman; Alkhuriji, Afrah F.; Metwally, Dina M.; Mahgoub, Shahenda; Amin, Hatem K.; Habotta, Ola A.; Abdel Moneim, Ahmed E.; Albeltagy, Rasha S.
• Is Part Of: Chemico-biological interactions. Volume 333(2021)
• Publication Date: 2021-01-05
• Subjects: Chlorogenic acid -- Sodium arsenite -- Reproductive toxicity -- Oxidative stress -- Apoptosis; CAT Catalase -- CGA Chlorogenic acid -- FSH Follicle-stimulating hormone -- GPx Glutathione peroxidase -- GR Glutathione reductase -- GSH Glutathione -- IL-1β Interleukin-1β -- LPO Lipid peroxidation -- LH Luteinizing hormone -- MDA Malondialdehyde -- NaAsO2 Sodium arsenite -- NF-κB Nuclear factor-κB Nuclear factor kappa B -- Nrf2 Nuclear factor erythroid 2-related factor 2 -- NO Nitric oxide -- RNS Reactive nitrogen species -- ROS Reactive oxygen species -- SOD Superoxide dismutase -- TNF-α Tumor necrosis factor-α; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2020.109333
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Arsenic, a major environmental pollutant of global concern, is well-known for its reproductive toxicity. In this study, the protective potential of chlorogenic acid (CGA), a caffeoylquinic acid isomer abundantly found in many plants, was investigated against sodium arsenite (NaAsO2 )-induced testicular dysfunctions. Adult male Swiss mice were either administered NaAsO2 alone at 5 mg kg −1 or co-treated with CGA at 100 mg kg −1 or 200 mg kg −1 body weight for 4 weeks. Results showed that NaAsO2 -treated mice exhibited marked declines in testes weight, sperm count, and viability accompanied by decreases in sexual hormonal levels. Moreover, NaAsO2 toxicity evoked exhaustion of antioxidant markers (SOD, CAT, GPx, GR, and GSH), down-regulation of Nrf2 (nuclear factor erythroid 2–related factor 2) gene expression level, and elevations in malondialdehyde. Further, elevations in inflammatory cytokines (IL-1, TNF-α, and IL-6) together with the up-regulation of pro-apoptotic biomarkers (Bax and caspase- 3) and down-regulation of anti-apoptotic Bcl-2 were observed in NaAsO2 intoxication. Immunohistochemical analysis of testis sections of NaAsO2 -treated mice showed high caspase-3 expression. These findings were well supported with testicular histopathological examination. However, pretreatment of mice with CGA resulted in noteworthy improvements in testicular damage induced by arsenic in a dose-dependent manner possibly mediated by the Nrf2 signaling pathway. Conclusively, CGA counteracted arsenic-induced testicular injury through its antioxidant, anti-inflammatory, and anti-apoptotic properties. Therefore, CGA could serve as a favorable intervention in the alleviation of arsenic-induced reproductive toxicity. Highlights: Exposure to arsenic causes apoptosis, inflammation and oxidative stress in testis. Chlorogenic acid inhibits arsenic-induced reproductive toxicity in rats. Chlorogenic acid has antioxidant, antiinflammatory and antiapoptotic activities.