Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach. (April 2021)

• Record Type: Journal Article
• Authors: Polloni, Lorena; Azevedo, Fernanda Van Petten Vasconcelos; Teixeira, Samuel Cota; Moura, Eloá; Costa, Tassia Rafaela; Gimenes, Sarah Natalie Cirilo; Correia, Lucas Ian Veloso; Freitas, Vitor; Yoneyama, Kelly Aparecida Geraldo; Rodrigues, Renata Santos; Lopes, Daiana Silva; Rodrigues, Veridiana de Melo
• Is Part Of: Toxicology in vitro. Volume 72(2021)
• Publication Date: 2021-04
• Subjects: PLA2 -- Bothrops pauloensis -- Antiangiogenic -- Cancer -- BnSP-7; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2021.105099
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Antiangiogenic strategies are promising tools for cancer treatment and several other disorders. In this sense, phospholipases A2 (PLA2 s) from snake venom have been described to possess antiangiogenic properties. In this study, we evaluated both in vitro and ex vivo antiangiogenic effects induced by BnSP-7, a Lys49 PLA2 isolated from Bothrops pauloensis snake venom. BnSP-7 was able to inhibit endothelial cell (HUVEC) proliferation, which was indeed confirmed by a modulation of cell cycle progression. Interestingly, BnSP-7 also inhibited the adhesion and migration of HUVECs and blocked in vitro angiogenesis in a VEGF-dependent manner, an important proangiogenic factor. Finally, BnSP-7 was capable of inhibiting sprouting angiogenic process through an ex vivo aortic ring assay. Taken together, these results indicate that BnSP-7 has potent in vitro and ex vivo antiangiogenic effect. Highlights: BnSP-7 inhibited cell cycle progression and colonies formation on HUVEC cells. BnSP-7 inhibited HUVEC cells migration and adhesion. BnSP-7 inhibited HUVEC cells vessel formation in vitro in VEGF-dependent manner. BnSP-7 inhibited microvessel sprouting ex vivo by mouse aortic ring assay.