TDFCAM: A method for estimating stable isotope trophic discrimination in wild populations. Issue 1 (6th January 2023)

• Record Type: Journal Article
• Authors: Johnson, Devin L.; Henderson, Michael T.; Franke, Alastair; Swan, George J. F.; McDonald, Robbie A.; Anderson, David L.; Booms, Travis L.; Williams, Cory T.
• Is Part Of: Ecology and evolution. Volume 13:Issue 1(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-06
• Subjects: animal diet -- Bayesian mixing model -- nest camera -- raptor -- stable isotope -- trophic discrimination factor -- trophic ecology; Ecology -- Periodicals; Evolution -- Periodicals; 577.05
• DOI: 10.1002/ece3.9709
• Languages: English
• ISSNs: 2045-7758

Abstract

Abstract: Stable isotope mixing models (SIMMs) are widely used for characterizing wild animal diets. Such models rely upon using accurate trophic discrimination factors (TDFs) to account for the digestion, incorporation, and assimilation of food. Existing methods to calculate TDFs rely on controlled feeding trials that are time‐consuming, often impractical for the study taxon, and may not reflect natural variability of TDFs present in wild populations. We present TDFCAM as an alternative approach to estimating TDFs in wild populations, by using high‐precision diet estimates from a secondary methodological source—in this case nest cameras—in lieu of controlled feeding trials, and provide a framework for how and when it should be applied. In this study, we evaluate the TDFCAM approach in three datasets gathered on wild raptor nestlings (gyrfalcons Falco rusticolus ; peregrine falcons Falco perigrinus ; common buzzards Buteo buteo ) comprising contemporaneous δ 13 C & δ 15 N stable isotope data and high‐quality nest camera dietary data. We formulate Bayesian SIMMs (BSIMMs) incorporating TDFs from TDFCAM and analyze their agreement with nest camera data, comparing model performance with those based on other relevant TDFs. Additionally, we perform sensitivity analyses to characterize TDFCAM variability, and identify ecological and physiological factors contributing to that variability in wild populations. Across species and tissue types, BSIMMs incorporating a TDFCAM outperformed any other TDF tested, producing reliable population‐level estimates of diet composition. We demonstrate that applying this approach even with a relatively low sample size ( n < 10 individuals) produced more accurate estimates of trophic discrimination than a controlled feeding study conducted on the same species. Between‐individual variability in TDFCAM estimates for ∆ 13 C & ∆ 15 N increased with analytical imprecision in the source dietary data (nest cameras) but was also explained by natural variables in the study population (e.g., nestling nutritional/growth status and dietary composition). TDFCAM is an effective method of estimating trophic discrimination in wild animal populations. Here, we use nest cameras as source dietary data, but this approach is applicable to any high‐accuracy method of measuring diet, so long as diet can be monitored over an interval contemporaneous with a tissue's isotopic turnover rate. Abstract : Trophic discrimination factors (TDFs) are an important component of stable isotope mixing models (and their use for characterizing wild animal diets) but are difficult to estimate under realistic conditions. We developed a method (TDFCAM) to estimate TDFs in wild populations using stable isotopes in conjunction with a secondary high‐precision diet estimate. We evaluated this approach in three wild raptor datasets, which had contemporaneous stable isotope and nest camera diet data, and found TDFcams to outperform any other TDF tested and produce reliable population‐level estimates of diet composition.