COVID-19 detection using AIE-active iridium complexes. Issue 5 (19th January 2023)

• Record Type: Journal Article
• Authors: Gupta, Ajay; Adarsh, Tarun; Manchanda, Vikas; Sasmal, Pijus K.; Gupta, Shalini
• Is Part Of: Dalton transactions. Volume 52:Issue 5(2023)
• Page Start: 1188
• Page End: 1192
• Publication Date: 2023-01-19
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/d2dt03554e
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : AIE-active turn-on iridium complexes attached to unique antisense oligonucleotide sequences have been developed for the selective detection of the SARS-CoV-2 virus. Abstract : The highly contagious COVID-19, caused by the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed using reverse transcription polymerase chain reaction (RT-PCR). However, despite being highly sensitive, RT-PCR is also time consuming and quite complex, which limits its use for point-of-care (POC) testing. We have developed a simple single-step fluorescence assay for SARS-CoV-2 RNA detection based on the principle of aggregation-induced emission (AIE) using iridium complexes. Our smartly designed iridium probes fluorescently "turn-on" in the presence of SARS-CoV-2 RNA and give specific results at room temperature within 10 min. The lower limit of detection (LOD) is 1.84 genome copies per reaction, and the sensitivity and specificity of the assay in 20 clinical samples are found to be 90% and 80%, respectively.