Expression of apolipoprotein A-I and A-II in rainbow trout reproductive tract and their possible role in antibacterial defence. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Dietrich, Mariola A.; Nynca, Joanna; Adamek, Mikołaj; Steinhagen, Dieter; Karol, Halina; Ciereszko, Andrzej
• Is Part Of: Fish & shellfish immunology. Volume 45:Issue 2(2015:Aug.)
• Page Start: 750
• Page End: 756
• Publication Date: 2015-08
• Subjects: Apolipoprotein -- Antimicrobial properties -- Seminal plasma -- Rainbow trout; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.05.048
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Antimicrobial proteins such as apolipoproteins A (ApoA-I and ApoA-II) play an important role in the primary defence barrier in vertebrates including fish. The aims of the present study were to isolate and characterise rainbow trout seminal plasma ApoA-I and ApoA-II, to examine the mRNA expression of each apolipoprotein in testis and spermatic ducts, and to test the antibacterial properties of the apolipoproteins. Using a three-step isolation procedure consisting of ion-exchange chromatography, gel filtration and preparative SDS-PAGE, apolipoproteins were purified and identified as ApoA-I and ApoA-II. Both apolipoproteins were represented by several proteoforms. The expression of ApoA-I and ApoA-II mRNA in the reproductive tract and their antibacterial properties against Escherichia coli suggest that seminal apolipoproteins play an important role in innate immunity in the rainbow trout reproductive tract. The functions of seminal ApoA can be related to protection of sperm and reproductive tissue from microbial attack and to the maintenance of sperm membrane integrity. Highlights: Apolipoproteins consist of several proteoforms and correspond to the major proteins of rainbow trout seminal plasma. mRNA expression of both apolipoproteins was detected in the testes and spermatic ducts. ApoA-I of seminal plasma displayed antibacterial activity.