Direct proof of the amyloid nature of yeast prions [PSI+] and [PIN+] by the method of immunoprecipitation of native fibrils. Issue 6 (31st August 2021)

• Record Type: Journal Article
• Authors: Sergeeva, Aleksandra V; Belashova, Tatyana A; Bondarev, Stanislav A; Velizhanina, Marya E; Barbitoff, Yury A; Matveenko, Andrew G; Valina, Anna A; Simanova, Angelina L; Zhouravleva, Galina A; Galkin, Alexey P
• Is Part Of: FEMS yeast research. Volume 21:Issue 6(2021)
• Publication Date: 2021-08-31
• Subjects: prion -- amyloid -- fibril immunoprecipitation -- Congo Red -- birefringence -- yeast; Yeast -- Periodicals; Yeasts -- Periodicals; 579.562
• DOI: 10.1093/femsyr/foab046
• Languages: English
• ISSNs: 1567-1356

Abstract

ABSTRACT: Prions are proteins that can exist in several structurally and functionally distinct states, one or more of which is transmissible. Yeast proteins Sup35 and Rnq1 in prion state ([ PSI + ] and [ PIN + ], respectively) form oligomers and aggregates, which are transmitted from parents to offspring in a series of generations. Several pieces of indirect evidence indicate that these aggregates also possess amyloid properties, but their binding to amyloid-specific dyes has not been shown in vivo . Meanwhile, it is the specific binding to the Congo Red dye and birefringence in polarized light after such staining that is considered the gold standard for proving the amyloid properties of a protein. Here, we used immunoprecipitation to extract native fibrils of the Sup35 and Rnq1 proteins from yeast strains with different prion status. These fibrils are detected by electron microscopy, stained with Congo Red and exhibit yellow-green birefringence after such staining. All these data show that the Sup35 and Rnq1 proteins in prion state form amyloid fibrils in vivo . The technology of fibrils extraction in combination with standard cytological methods can be used to identify new pathological and functional amyloids in any organism and to analyze the structural features of native amyloid fibrils. Abstract : The method of immunoprecipitation of native fibrils allows to obtain direct evidence of the amyloid nature of yeast prions [PSI + ] and [PIN + ] and can be used to identify new amyloids.