Direct amplification of reference samples with Globalfiler® PCR Amplification Kit. (December 2015)

• Record Type: Journal Article
• Authors: Gouveia, N.; Brito, P.; Serra, A.; Balsa, F.; Andrade, L.; Bento, M.São; Cunha, P.; Bogas, V.; Lopes, V.; Porto, M.J.
• Is Part Of: Forensic science international. Volume 5(2015)
• Page Start: e135
• Page End: e137
• Publication Date: 2015-12
• Subjects: GlobalFiler -- Direct amplification -- Reference samples; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2015.09.055
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: The GlobalFiler ® PCR Amplification system uses 6-dye fluorescent chemistry to enable multiplexing of 21 autosomal STRs, 1 Y-STR, 1 Y-indel and the sex-determining marker amelogenin. Validation studies were conducted to assess the performance of the GlobalFiler ® casework kit for typing reference samples through a direct amplification. The findings support that the GlobalFiler ® PCR Amplification Kit produces reliable results from blood and buccal reference DNA samples.