GABAergic Neurons in the Dorsal Raphe Nucleus that Express 5-HT3A Receptors Participate in Responses to Stress Hormones. (10th August 2020)

• Record Type: Journal Article
• Authors: Li, Xiaotao; Chen, Shanping; Yang, Haiyang; Li, Xiang; So, Kwok-Fai; Wang, Liping
• Is Part Of: Neuroscience. Volume 441(2020)
• Page Start: 217
• Page End: 225
• Publication Date: 2020-08-10
• Subjects: DRI interfascicular part of dorsal raphe nucleus -- DRN dorsal raphe nucleus -- DRV ventral part of dorsal raphe nucleus -- DRVL ventrolateral part of dorsal raphe nucleus -- GABA gamma-aminobutyric acid -- PV parvalbumin -- SOM somatostatin -- SSRIs selective serotonin reuptake inhibitors; dorsal raphe nucleus -- serotonin -- GABA -- 5HT3AR -- stress -- corticosterone; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2020.05.055
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Highlights: The 5HT3A positive cells were predominated the GABA cells at rat DRN, accounted for 97.5%. While the population of PV+ and SOM+ cells were sparse at the rat DRN. Those 5HT3A + GABA cells were more sensitive to stress hormone increase than the 5-HT cells at DRN. Abstract: The dorsal raphe nucleus (DRN) participates in stress responses and in mood regulation via its ascending release of serotonin (5-HT) onto neural circuits within the forebrain. Although the 5-HT DRN region is easily defined via 5-HT-expressing DRN neurons, the neuroarchitecture and microcircuitry that confer its multifunctionality have remained incompletely understood and have required further investigation. In this present study, neurochemical interactions within different subregions of the rat DRN were precisely analyzed. We found that 97.5% of GABAergic neurons in the DRN expressed ionotropic 5-HT3A receptors (5-HT3ARs), whereas there were only rare parvalbumin (PV)-positive or somatostatin (SOM)-positive GABAergic neurons. Furthermore, corticosterone administration into male rats as a rodent model of depression induced significantly higher c-Fos expression in 5-HT3AR-positive GABAergic neurons compared to that in 5-HT neurons within the DRN. Taken together, our findings suggest that 5-HT3AR-positive GABAergic neurons in the DRN participate in responses to stress hormones in a rat model of depression.