Molecular and functional roles of 6C CC chemokine 19 in defense system of striped murrel Channa striatus. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Arockiaraj, Jesu; Bhatt, Prasanth; Harikrishnan, Ramasamy; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah
• Is Part Of: Fish & shellfish immunology. Volume 45:Issue 2(2015:Aug.)
• Page Start: 817
• Page End: 827
• Publication Date: 2015-08
• Subjects: Channa striatus -- Chemokine -- Cellular proliferation -- Chemotaxis -- Respiratory burst activity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.06.001
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In this study, we have reported the molecular information of chemokine-19 (Chem19) from striped murrel Channa striatus ( Cs ). Cs CC-Chem19 cDNA sequence was 555 base pair (bp) in length which is 68 bp 5′ untranslated region (UTR), 339 bp translated region and 149 bp 3′ UTR. The translated region is encoded for a polypeptide of 112 amino acids. Cs CC-Chem19 peptide contains a signal sequence between 1 and 26 and an interleukin (IL) 8 like domain between 24 and 89. The multiple sequence alignment showed a 'DCCL' motif, an indispensable motif present in all CC chemokines which was conserved throughout the evolution. Phylogenetic tree showed that Cs CC-Chem19 formed a cluster with chemokine 19 from fishes. Secondary structure of Cs CC-Chem19 revealed that the peptide contains maximum amount of coils (61.6%) compared to α-helices (25.9%%) and β-sheet (12.5%). Further, 3D analysis indicated that the cysteine residues at 33, 34, 59 and 75 making the disulfide bridges as 33 = 59 and 34 = 75. Significantly (P < 0.05) highest Cs CC-Chem19 mRNA expression was observed in blood and it was up-regulated upon fungus and bacterial infection. Utilizing the coding region of Cs CC-Chem19, recombinant CsCC-Chem19 protein was produced. The recombinant Cs CC-Chem19 protein induced the cellular proliferation and respiratory burst activity of C. striatus peripheral blood leukocytes (PBL) in a concentration dependent manner. Moreover, the chemotactic activity showed that the recombinant Cs CC-Chem19 significantly (P < 0.05) enhanced the movement of PBL of C. striatus . Conclusively, Cs CC-Chem19 is a 6C CC chemokine having an ability to perform both inflammatory and homeostatic functions. However, further research is necessary to understand the potential of 6C CC chemokine 19 of C. striatus, particularly their regulatory ability on different cellular components in the defense system. Highlights: Murrel 6C CC chemokine-19 characterized at molecular level. Cs CC-Chem19 contains an interleukin (IL) 8 like domain. Cs CC-Chem19 was highly expressed in blood upon microbial pathogen. Recombinant Cs CC-Chem19 induced cellular proliferation and respiratory burst. Cs CC-Chem19 chemotaxis enhanced the movement of Channastriatus PBL.