Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish. (1st March 2021)

• Record Type: Journal Article
• Authors: Zheng, Yuanru; Tian, Chunyang; Fan, Chunlin; Xu, Nishan; Xiao, Junjie; Zhao, Xiaoyang; Lu, Zibin; Cao, Huihui; Liu, Junshan; Yu, Linzhong
• Is Part Of: Journal of ethnopharmacology. Volume 267(2021)
• Publication Date: 2021-03-01
• Subjects: Sheng-Mai Yin -- LPS -- Anti-inflammatory -- RAW 264.7 cell -- Zebrafish; ANOVA analysis of variance -- Dex dexamethasone -- DMSO dimethyl sulphoxide -- dpf days post fertilization -- ELISA enzyme-linked immunosorbent assay -- GFP green fluorescent protein -- H&E hematoxylin and eosin -- hpi hours post injection -- HPLC high-performance liquid chromatography -- IκBα inhibitor of kappa B -- IL-6 interleukin-6 -- LPS lipopolysaccharide -- MPO myeloperoxidase -- MTT 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazoliumbromide -- NF-κB nuclear factor-kappa B -- PBS phosphate-buffered saline -- qRT-PCR quantitative real-time polymerase chain reaction -- SEM standard error of mean -- SMY Sheng-Mai Yin -- STAT3 signal transducer and activator of transcription 3 -- TNF-α tumor necrosis factor-α -- BCA bicinchoninic acid -- DAPI 4′, 6-diamidino-2-phenylindole -- ECL enhanced chemiluminescence -- Ser serine -- Tyr tyrosine; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113497
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Sheng-Mai Yin (SMY), a famous traditional Chinese medicine formula, has been commonly used in China for centuries to treat various diseases, such as inflammation-related diseases. However, the anti-inflammatory activity of SMY and its potential mechanisms still have not yet been clearly understood. Aim of the study : In this study, we aimed to determine the anti-inflammatory effect of SMY and explore its underlying mechanisms both on RAW 264.7 cells and zebrafish. Materials and methods: The levels of pro-inflammatory cytokines IL-6 and TNF-α secreted by RAW 264.7 cells were measured by ELISA. The protein expressions of IκBα, p-IκBα (Ser32), STAT3 and p-STAT3 (Tyr705) were determined by Western blotting. And the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 macrophage cells was detected by confocal microscopy. Moreover, the in vivo anti-inflammatory effect of SMY and its potential mechanisms were further investigated by survival analysis, hematoxylin-eosin staining (H&E), observation of neutrophil migration and quantitative real-time PCR (qRT-PCR) analysis in zebrafish inflammatory models. Results: SMY reduced the release of IL-6 and TNF-α, inhibited the phosphorylation of IκBα and STAT3 as well as the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 cells. Furthermore, the increased survival, decreased infiltration of inflammatory cells and the attenuated migration of neutrophils together suggested the in vivo anti-inflammatory effects of SMY. More importantly, SMY reduced the gene expressions of pro-inflammatory cytokines and suppressed LPS-induced up-regulation of NF-κB, IκBα and STAT3 in zebrafish inflammatory models. Conclusion: SMY exerts significant anti-inflammatory effects with a potential mechanism of inhibiting the NF-κB and STAT3 signal pathways. Our findings suggest a scientific rationale of SMY to treat inflammatory diseases in clinic. Graphical abstract: Image 1