3D Gelatin Microsphere Scaffolds Promote Functional Recovery after Spinal Cord Hemisection in Rats. Issue 3 (1st December 2022)

• Record Type: Journal Article
• Authors: Ke, Hongfei; Yang, Hongru; Zhao, Yijing; Li, Tingting; Xin, Danqing; Gai, Chengcheng; Jiang, Zige; Wang, Zhen
• Is Part Of: Advanced science. Volume 10:Issue 3(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-01
• Subjects: axon regeneration -- functional recovery -- gelatin microspheres scaffolds -- neuroinflammation -- spinal cord injury; Science -- Periodicals; 505
• DOI: 10.1002/advs.202204528
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Spinal cord injury (SCI) damages signal connections and conductions, with the result that neuronal circuits are disrupted leading to neural dysfunctions. Such injuries represent a serious and relatively common central nervous system condition and current treatments have limited success in the reconstruction of nerve connections in injured areas, especially where sizeable gaps are present. Biomaterial scaffolds have become an effective alternative to nerve transplantation in filling these gaps and provide the foundation for simulating the 3D structure of solid organs. However, there remain some limitations with the application of 3D bioprinting for preparation of biomaterial scaffolds. Here, the approach in constructing and testing mini‐tissue building blocks and self‐assembly, solid 3D gelatin microsphere (GM) scaffolds with multiple voids as based on the convenient preparation of gelatin microspheres by microfluidic devices is described. These 3D GM scaffolds demonstrate suitable biocompatibility, biodegradation, porosity, low preparation costs, and relative ease of production. Moreover, 3D GM scaffolds can effectively bridge injury gaps, establish nerve connections and signal transductions, mitigate inflammatory microenvironments, and reduce glial scar formation. Accordingly, these 3D GM scaffolds can serve as a novel and effective bridging method to promote nerve regeneration and reconstruction and thus recovery of nerve function after SCI. Abstract : 3D gelatin microsphere scaffolds with suitable void size, biocompatibility, porosity, and degradability are fabricated by a microfluidic device with low cost and facile process. It is used to bridge injury gaps in rats. After spinal cord injury, its unique porous structure improves the microenvironment, promotes the survival and regeneration of neurons and axons, promoting functional recovery after spinal cord injury.