Overview of Digital Electrophoresis Analysis. Issue 1 (23rd January 2023)

• Record Type: Journal Article
• Authors: Wang, Qi; Chang, Neil; Moomaw, Butch; Medberry, Scott; Gallagher, Sean R.
• Is Part Of: Current protocols. Volume 3:Issue 1(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-23
• Subjects: analysis -- CCD -- 2D PAGE -- electrophoresis -- imaging -- sCMOS; Life sciences -- Laboratory manuals -- Periodicals; Biology -- Laboratory manuals -- Periodicals; Life sciences -- Technique -- Periodicals; Biology -- Technique -- Periodicals; 570.028
• DOI: 10.1002/cpz1.651
• Languages: English
• ISSNs: 2691-1299

Abstract

Abstract: Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using sCMOS and CCD‐based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of technologies used for image capture, and provides an introduction to the software and methods used for one‐ and two‐dimensional digital image analysis. © 2023 Wiley Periodicals LLC.