Berberine inhibits RA-FLS cell proliferation and adhesion by regulating RAS/MAPK/FOXO/HIF-1 signal pathway in the treatment of rheumatoid arthritis. Issue 2 (1st February 2023)

• Record Type: Journal Article
• Authors: Li, Zhiqi; Chen, Meilin; Wang, Zhaoyi; Fan, Qiqi; Lin, Zili; Tao, Xiaoyu; Wu, Jiarui; Liu, Zhenquan; Lin, Ruichao; Zhao, Chongjun
• Is Part Of: Bone & joint research. Volume 12:Issue 2(2023)
• Page Start: 91
• Page End: 102
• Publication Date: 2023-02-01
• Subjects: Berberine -- Traditional Chinese medicine -- Rheumatoid arthritis -- rheumatoid arthritis -- fibroblast-like synoviocytes -- MMP-3 -- Western blot -- hypoxia -- quantitative polymerase chain reaction -- Enzyme-linked immunosorbent assay -- matrix metalloproteinases (MMPs) -- arthritis -- polymerase chain reaction; Musculoskeletal system -- Periodicals; 573.705
• DOI: 10.1302/2046-3758.122.BJR-2022-0269.R1
• Languages: English
• ISSNs: 2046-3758

Abstract

Abstract : Aims: Rheumatoid arthritis (RA) is a common chronic immune disease. Berberine, as its main active ingredient, was also contained in a variety of medicinal plants such as Berberaceae, Buttercup, and Rutaceae, which are widely used in digestive system diseases in traditional Chinese medicine with anti-inflammatory and antibacterial effects. The aims of this article were to explore the therapeutic effect and mechanism of berberine on rheumatoid arthritis. Methods: Cell Counting Kit-8 was used to evaluate the effect of berberine on the proliferation of RA fibroblast-like synoviocyte (RA-FLS) cells. The effect of berberine on matrix metalloproteinase (MMP)-1, MMP-3, receptor activator of nuclear factor kappa-Β ligand (RANKL), tumour necrosis factor alpha (TNF-α), and other factors was determined by enzyme-linked immunoassay (ELISA) kit. Transcriptome technology was used to screen related pathways and the potential targets after berberine treatment, which were verified by reverse transcription-polymerase chain reaction (RT-qPCR) and Western blot (WB) technology. Results: Berberine inhibited proliferation and adhesion of RA-FLS cells, and significantly reduced the expression of MMP-1, MMP-3, RANKL, and TNF-α. Transcriptional results suggested that berberine intervention mainly regulated forkhead box O (FOXO) signal pathway, prolactin signal pathway, neurotrophic factor signal pathway, and hypoxia-inducible factor 1 (HIF-1) signal pathway. Conclusion: The effect of berberine on RA was related to the regulation of RAS/mitogen-activated protein kinase/FOXO/HIF-1 signal pathway in RA-FLS cells. Cite this article: Bone Joint Res 2023;12(2):91–102.