Frequent Assembly of Chimeric Complexes in the Protein Interaction Network of an Interspecies Yeast Hybrid. (30th November 2020)

• Record Type: Journal Article
• Authors: Dandage, Rohan; Berger, Caroline M; Gagnon-Arsenault, Isabelle; Moon, Kyung-Mee; Stacey, Richard Greg; Foster, Leonard J; Landry, Christian R
• Editors: Meaux, Juliette de
• Is Part Of: Molecular biology and evolution. Volume 38:Number 4(2021)
• Page Start: 1384
• Page End: 1401
• Publication Date: 2020-11-30
• Subjects: protein–protein interaction -- hybridization -- Saccharomyces cerevisiae -- Saccharomyces uvarum; Molecular biology -- Periodicals; Molecular evolution -- Periodicals; Evolution, Molecular -- Periodicals; Molecular Biology -- Periodicals; 572.8
• DOI: 10.1093/molbev/msaa298
• Languages: English
• ISSNs: 0737-4038

Abstract

Abstract: Hybrids between species often show extreme phenotypes, including some that take place at the molecular level. In this study, we investigated the phenotypes of an interspecies diploid hybrid in terms of protein–protein interactions inferred from protein correlation profiling. We used two yeast species, Saccharomyces cerevisiae and Saccharomyces uvarum, which are interfertile, but yet have proteins diverged enough to be differentiated using mass spectrometry. Most of the protein–protein interactions are similar between hybrid and parents, and are consistent with the assembly of chimeric complexes, which we validated using an orthogonal approach for the prefoldin complex. We also identified instances of altered protein–protein interactions in the hybrid, for instance, in complexes related to proteostasis and in mitochondrial protein complexes. Overall, this study uncovers the likely frequent occurrence of chimeric protein complexes with few exceptions, which may result from incompatibilities or imbalances between the parental proteomes.