Bioassay-guided isolation of antiplasmodial and antimicrobial constituents from the roots of Terminalia albida. (1st March 2021)

• Record Type: Journal Article
• Authors: Baldé, Mamadou Aliou; Tuenter, Emmy; Matheeussen, An; Traoré, Mohamed Sahar; Cos, Paul; Maes, Louis; Camara, Aïssata; Diallo, Mamadou Saliou Telly; Baldé, Elhadj Saïdou; Balde, Aliou Mamadou; Pieters, Luc; Foubert, Kenn
• Is Part Of: Journal of ethnopharmacology. Volume 267(2021)
• Publication Date: 2021-03-01
• Subjects: Terminalia albida -- Combretaceae -- Antiplasmodial activity -- Triterpenes -- Pantolactone; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113624
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Terminalia albida (Combretaceae), widely used in Guinean traditional medicine, showed promising activity against Plasmodium falciparum and Candida albicans in previous studies. Bioassay-guided fractionation was carried out in order to isolate the compounds responsible for these activities. Materials and methods: Fractionation and isolation were performed by flash chromatography, followed by semi-preparative HPLC-DAD-MS. The structural elucidation of the isolated compounds was carried out by 1D and 2D NMR as well as HR-ESI-MS. Isolated compounds were evaluated against Plasmodium falciparum, Candida albicans, Staphylococcus aureus and Escherichia coli, and their cytotoxicity against MRC-5 cells was determined. Results: Bioassay-guided fractionation of Terminalia albida root resulted in the isolation of 14 compounds (1 –14 ), and their antimicrobial properties were evaluated. Pantolactone (1 ) (IC50 0.60 ± 0.03 μM) demonstrated significant activity against P. falciparum. Other compounds, including 3, 4, 3′-tri-O-methyl-ellagic acid (3), the triterpenes arjunolic acid (5), arjungenin (6), arjunic acid (7) and arjunglucoside II (10), and the phenol glycoside calophymembranside-B (14 ), were less active and showed IC50 values in the range 5–15 μM. None of the tested compound showed antibacterial or antifungal activity . Conclusion: These results may explain at least in part the activity of the root extract of T. albida against P. falciparum . Graphical abstract: Image 1