Dependence of visual and cognitive outcomes on animal holder configuration in a rodent model of blast overpressure exposure. (November 2021)

• Record Type: Journal Article
• Authors: Allen, Rachael S.; Motz, Cara T.; Singh, Anayesha; Feola, Andrew; Hutson, Lauren; Douglass, Amber; Ramachandra Rao, Sriganesh; Skelton, Lara A.; Cardelle, Lidia; Bales, Katie L.; Chesler, Kyle; Gudapati, Kaavya; Ethier, C. Ross; Harper, Matthew M.; Fliesler, Steven J.; Pardue, Machelle T.
• Is Part Of: Vision research. Volume 188(2021)
• Page Start: 162
• Page End: 173
• Publication Date: 2021-11
• Subjects: Blast -- Traumatic brain injury -- TBI -- Retina -- Eye; Vision -- Periodicals; 573.88
• DOI: 10.1016/j.visres.2021.07.008
• Languages: English
• ISSNs: 0042-6989

Abstract

Highlights: Type of animal holder used in blast studies affects visual and cognitive outcomes. Enclosed holders may cause secondary damage to the contralateral eye. Damage may involve concussive injury or blast wave reflection off the holder wall. Open holders may damage the brain due to head movement (whiplash). Effects other than the primary blast wave should be considered post-blast exposure. Abstract: Blast-induced traumatic brain injury is the signature injury of modern military conflicts. To more fully understand the effects of blast exposure, we placed rats in different holder configurations, exposed them to blast overpressure, and assessed the degree of eye and brain injury. Anesthetized Long-Evans rats received blast exposures directed at the head (63 kPa, 195 dB-SPL) in either an "open holder" (head and neck exposed; n = 7), or an "enclosed holder" (window for blast exposure to eye; n = 15) and were compared to non-blast exposed (control) rats (n = 22). Outcomes included optomotor response (OMR), electroretinography (ERG), and spectral domain optical coherence tomography (SD-OCT) at 2, 4, and 6 months post-blast, and cognitive function (Y-maze) at 3 months. Spatial frequency and contrast sensitivity were reduced in ipsilateral blast-exposed eyes in both holders, while contralateral eyes showed greater deficits with the enclosed holder. Thinner retinas and reduced ERG a- and b- wave amplitudes were observed for both ipsilateral and contralateral eyes with the enclosed, but not the open, holder. Rats in the open holder showed cognitive deficits compared to rats in the enclosed holder. Overall, the animal holder configuration used in blast exposure studies can significantly affect outcomes. Enclosed holders may cause secondary damage to the contralateral eye by concussive injury or blast wave reflection off the holder wall. Open holders may damage the brain via rapid head movement (contrecoup injury). These results highlight additional factors to be considered when evaluating patients with blast exposure or developing models of blast injury.