Comparison between mixed lysate antigen and α-actinin antigen in ELISA for serodiagnosis of trichomoniasis. Issue 5 (October 2015)

• Record Type: Journal Article
• Authors: Kim, Seung-Ryong; Kim, Jung-Hyun; Park, Soon-Jung; Lee, Hye-Yeon; Kim, Yong-Suk; Kim, Yu-Mi; Hong, Yeon-Chul; Ryu, Jae-Sook
• Is Part Of: Parasitology international. Volume 64:Issue 5(2015:Oct.)
• Page Start: 405
• Page End: 407
• Publication Date: 2015-10
• Subjects: Trichomonas vaginalis -- Lysate -- Recombinant α-actinin -- ELISA -- Serodiagnosis; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2015.06.003
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: The aim of this study was to identify an antigen suitable for ELISA for serodiagnosis of Trichomonas vaginalis ( T. vaginalis ) infection. Mixed lysate antigen (Ag) from eight strains of T . vaginalis and recombinant α-actinin protein was compared. The sera of three groups were examined by ELISA: 73 women infected with trichomoniasis served as a positive control, 31 male volunteers as a negative control, and 424 women attending an outpatient health screening at Hanyang University Guri Hospital. Based on the cutoff optical density for each Ag obtained with a negative control, the serosensitivity of the mixed lysate Ag (79.5%) was significantly higher than that of the α-actinin (52.1%) in the 73 patients with trichomoniasis. The specificity using lysate Ag and α-actinin was 100% and 96.8%, respectively. On the other hand, the positivity rate in 424 outpatients was 39.2% and 11.8% with mixed lysate and α-actinin Ag, respectively. Taken together, mixed lysate Ag showed higher sensitivity and specificity than α-actinin. Therefore, mixed lysate may be a better Ag than α-actinin for ELISA for the diagnosis of trichomoniasis. Graphical abstract: Highlights: We compared mixed lysate and α-actinin as ELISA Ag for the serodiagnosis of T. vaginalis infection. The mixed lysate may be a better Ag than α-actinin.