A custom software solution for forensic mtDNA analysis of MiSeq data. (December 2015)

• Record Type: Journal Article
• Authors: Holland, Mitchell; McElhoe, Jennifer
• Is Part Of: Forensic science international. Volume 5(2015)
• Page Start: e614
• Page End: e616
• Publication Date: 2015-12
• Subjects: NextGENe® -- Heteroplasmy -- Length-variants -- DNA motifs; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2015.09.242
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: The use of a next generation sequencing (NGS) approach for mitochondrial (mt) DNA analysis in forensic casework is imminent. A feature of the NGS approach is the ability to detect and report mtDNA heteroplasmy, which will significantly enhance the discrimination potential of the testing method. A single software solution that allows for robust and user-friendly analysis of NGS-derived mtDNA sequence is not readily available, especially when considering heteroplasmy. This communication outlines the desired features of a software package for forensic applications, and the progress made towards those aims with the development of a custom version of NextGENe ® .