Molecular cloning, characterization, and expression analysis of two different types of lectins from the oriental river prawn, Macrobrachium nipponense. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Xiu, Yunji; Wang, Yinghui; Jing, Yunting; Qi, Yakun; Ding, Zhengfeng; Meng, Qingguo; Wang, Wen
• Is Part Of: Fish & shellfish immunology. Volume 45:Issue 2(2015:Aug.)
• Page Start: 465
• Page End: 469
• Publication Date: 2015-08
• Subjects: Macrobrachium nipponense -- L-Type lectin -- M-Type lectin -- Pattern recognition protein -- Immune response; MnMTL1 M-type lectin of Macrobrachium nipponense -- MnLTL1 L-type lectin of Macrobrachium nipponense -- GH47 Glycoside hydrolase family 47 -- proPO prophenoloxidase -- PRPs Pattern recognition proteins -- PAMP pathogen associated molecular patterns -- CRD carbohydrate recognition domain; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.04.022
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Lectins, which are widely expressed in invertebrates, play important roles in many biological processes, including protein trafficking, cell signaling, pathogen recognition, as effector molecules, and so on (Wang and Wang, 2013). This study identified one novel M-type lectin and one L-Type lectin, designated as MnMTL1 and MnLTL1, from the oriental river prawn Macrobrachium nipponense . The full-length cDNA of MnMTL1 was 2064 bp with a 1761 bp ORF encoding a putative protein of 586 deduced amino acids. The full-length cDNA of MnLTL1 was 1744 bp with a 972 bp ORF encoding a 323-amino acid peptide. The deduced MnMTL1 protein contained a putative type II transmembrane region and a 440-aa Glycoside hydrolase family 47 (GH47) domain. One luminal carbohydrate recognition domain and a 23-aa type I transmembrane region were identified from the MnLTL1. MnMTL1 shared 78% identity with Marsupenaeus japonicus M-type lectin and MnLTL1 shared 83% similarity with M. japonicus L-type lectin. RT-PCR analysis showed that MnMTL1 and MnLTL1 were expressed in all tested tissues. Quantitative real-time PCR analysis revealed that MnMTL1 and MnLTL1 are substantially fluctuant during Aeromonas hydrophila and Aeromonas veronii infections. Based on immune responses and previous literature, we assumed that MnMTL1 and MnLTL1 might be functioned as pattern recognition receptors and play important roles in the immune response of M. nipponense . Highlights: Two novel lectins (MnMTL1, MnLTL1) were identified from Macrobrachium nipponense . The nucleotide and amino acid sequences were characterized and compared. The expression profiles of MnMTL1 and MnLTL1 in various tissues were investigated. The both transcripts were studied after challenge with Aeromonas hydrophila and Aeromonas veronii .