Mesenchymal Stem Cell Preparation and Transfection‐free Ferumoxytol Labeling for MRI Cell Tracking. (13th February 2018)

• Record Type: Journal Article
• Authors: Liu, Li; Ho, Chien
• Editors: Bhatia, Mick; Elefanty, Andrew G.; Fisher, Susan J.; Patient, Roger; Schlaeger, Thorsten; Snyder, Evan Y.
• Is Part Of: Current protocols in stem cell biology. Volume 43(2018)
• Page Start: 2B.7.1
• Page End: 2B.7.14
• Publication Date: 2018-02-13
• Subjects: Ferumoxytol (Feraheme®) -- magnetic resonance imaging -- stem cell culture and expansion -- stem cell tracking -- mesenchymal stem cells; Stem cells -- Laboratory manuals; Stem cells -- Physiology; Stem Cells; Stem cells; Laboratory Manual; Laboratory manuals; 571.5
• DOI: 10.1002/cpsc.38
• Languages: English
• ISSNs: 1941-7322

Abstract

Abstract: Mesenchymal stem cells (MSCs) are multipotent cells and are the most widely studied cell type for stem cell therapies. In vivo cell tracking of MSCs labeled with an FDA‐approved superparamagnetic iron‐oxide (SPIO) particle by magnetic resonance imaging (MRI) provides essential information, e.g., MSC engraftment, survival, and fate, thus improving cell therapy accuracy. However, current methodology for labeling MSCs with Ferumoxytol (Feraheme ® ), the only FDA‐approved SPIO particle, needs transfection agents. This unit describes a new "bio‐mimicry" protocol to prepare more native MSCs by using more " in vivo environment" of MSCs, so that the phagocytic activity of cultured MSCs is restored and expanded MSCs can be labeled with Ferumoxytol, without the need for transfection agents and/or electroporation. Moreover, MSCs re‐size to a more native size, reducing from 32.0 to 19.5 μm. The MSCs prepared from this protocol retain more native properties and would be useful for biomedical applications and MSC‐tracking studies by MRI. © 2017 by John Wiley & Sons, Inc.